terça-feira, 29 de dezembro de 2009
domingo, 27 de dezembro de 2009
Infectonator: Christmas Edition
Pra quem tá no tédio ai vai o joguinho feliz de matar pessoas:
http://www.freewebarcade.com/game/infectonator-christmas-edition/
segunda-feira, 21 de dezembro de 2009
Notícia animal
Em recente conversa na cantina, fico sabendo de mais um ataque de tubarão na cidade de Recife. Desta vez, o ataque foi desferido sobre as nádegas de um banhista.
A notícia foi veiculada em jornal de grande circulação:
A notícia foi veiculada em jornal de grande circulação:
Testemunhas oculares afirmam ter visto a besta. Segue imagem capturada por reconstituição 3D, a partir de descrição dos transeuntes.
terça-feira, 15 de dezembro de 2009
quarta-feira, 9 de dezembro de 2009
Sensacional!!
Você, estudante de iniciação científica/pós-graduação, que trabalha intensamente com biologia molecular e vê TODOS OS SEUS EXPERIMENTOS DANDO ERRADO, não se preocupe mais.
Recentemente, foi publicado na revista Journal of Biomolecular Techniques (JBT), no portal PubMed, um artigo totalmente mirabolante, mas extremamente útil.
Segue abaixo o resumo...
J Biomol Tech. 2009 Dec;20(5):236-40.
An assessment of air as a source of DNA contamination encountered when performing PCR.
Witt N, Rodger G, Vandesompele J, Benes V, Zumla A, Rook GA, Huggett JF.
Centre for Infectious Diseases and International Health, Windeyer Institute for Medical Sciences, University College London, London, United Kingdom.
Sensitive molecular methods, such as the PCR, can detect low-level contamination, and careful technique is required to reduce the impact of contaminants. Yet, some assays that are designed to detect high copy-number target sequences appear to be impossible to perform without contamination, and frequently, personnel or laboratory environment are held responsible as the source. This complicates diagnostic and research analysis when using molecular methods. To investigate the air specifically as a source of contamination, which might occur during PCR setup, we exposed tubes of water to the air of a laboratory and clean hood for up to 24 h. To increase the chances of contamination, we also investigated a busy open-plan office in the same way. All of the experiments showed the presence of human and rodent DNA contamination. However, there was no accumulation of the contamination in any of the environments investigated, suggesting that the air was not the source of contamination. Even the air from a busy open-plan office was a poor source of contamination for all of the DNA sequences investigated (human, bacterial, fungal, and rodent). This demonstrates that the personnel and immediate laboratory environment are not necessarily to blame for the observed contamination.
PMID: 19949694 [PubMed - in process]
A partir de hoje, qualquer erro de contaminação será responsabilidade do AR, e não do espirro que você deu em cima das suas amostras.
Recentemente, foi publicado na revista Journal of Biomolecular Techniques (JBT), no portal PubMed, um artigo totalmente mirabolante, mas extremamente útil.
Segue abaixo o resumo...
__________________________________
J Biomol Tech. 2009 Dec;20(5):236-40.
An assessment of air as a source of DNA contamination encountered when performing PCR.
Witt N, Rodger G, Vandesompele J, Benes V, Zumla A, Rook GA, Huggett JF.
Centre for Infectious Diseases and International Health, Windeyer Institute for Medical Sciences, University College London, London, United Kingdom.
Sensitive molecular methods, such as the PCR, can detect low-level contamination, and careful technique is required to reduce the impact of contaminants. Yet, some assays that are designed to detect high copy-number target sequences appear to be impossible to perform without contamination, and frequently, personnel or laboratory environment are held responsible as the source. This complicates diagnostic and research analysis when using molecular methods. To investigate the air specifically as a source of contamination, which might occur during PCR setup, we exposed tubes of water to the air of a laboratory and clean hood for up to 24 h. To increase the chances of contamination, we also investigated a busy open-plan office in the same way. All of the experiments showed the presence of human and rodent DNA contamination. However, there was no accumulation of the contamination in any of the environments investigated, suggesting that the air was not the source of contamination. Even the air from a busy open-plan office was a poor source of contamination for all of the DNA sequences investigated (human, bacterial, fungal, and rodent). This demonstrates that the personnel and immediate laboratory environment are not necessarily to blame for the observed contamination.
PMID: 19949694 [PubMed - in process]
__________________________________
A partir de hoje, qualquer erro de contaminação será responsabilidade do AR, e não do espirro que você deu em cima das suas amostras.
terça-feira, 24 de novembro de 2009
domingo, 22 de novembro de 2009
quinta-feira, 12 de novembro de 2009
Assinar:
Postagens (Atom)